芥蓝游离小孢子培养初报

 以芥蓝的5个基因型为试材，对游离小孢子培养诱导胚状体发生及植株再生进行了初步探讨。结果表明：供体基因型对成功诱导胚状体发生至关重要；振荡培养明显有利于胚状体的发育；改良MS和B培养基对诱导植株再生没有差别。     

稀土元素铈对‘红地球’葡萄组培苗生长的影响

 以‘红地球’葡萄组培苗茎段为外植体，在培养基中添加1～100 mg·L^-1 Ce(NO₃)₃，，研究 不同浓度Ce(NO₃)₃对其生长的影响。结果表明适宜浓度的Ce(NO，)，(1～10 mg·L^-1 )对‘红地球’葡萄组培苗的生长具有促进作用，加快外植体生根，侧根数及侧根总长显著增加,根、茎、叶鲜样质量和干样质量也增加，根系活力提高而增加移栽成活率，增加叶绿素含量，有利于植株对矿质营养的吸收。而100 mg·L^-1。Ce(NO₃)₃对葡萄组培苗生长起明显抑制作用。     

‘罗田甜柿’胚乳培养获得十二倍体再生植株

 取‘罗田甜柿’自然授粉后约70 d 的胚乳, 采用不同细胞分裂素和生长素组合进行离体培养。结果表明: 以1/ 2MS (1/ 2N ) + ZT (玉米素) 1.1 mg/ L + 2,4-D 1.0 mg/ L + CH (水解酪蛋白) 1000mg/ L 诱导产生的愈伤组织易获得再生植株; 形态学和细胞学检测确认再生植株中存在2n=12x=180 的个体。该十二倍体植株对中国原产甜柿的遗传改良可能具有潜在的应用价值。     

不同培养条件对鲍鱼菇菌丝生长的影响

通过不同温度、pH值及培养基含水量处理 ,对鲍鱼菇菌丝生长状况进行了研究。结果表明鲍鱼菇菌丝生长适宜的温度范围为 2 0～ 31℃ ,最适温度为 2 5～2 8℃ ;pH值适宜的范围为 4 0～ 7 0 ,最适pH值为 5 5～6 0 ;培养基含水量适宜的范围为 6 0 %～ 80 % ,最适含水量为 80 %。     

基于GIS的祁连山森林景观格局分析

在地理信息系统软件ArcGIS环境下 ,将祁连山区DEM图和坡向图分别同植被图叠加 ,分析研究区各景观组分在空间的分布特征 ;用定量分析景观结构和景观格局程序Fragstats计算景观和各景观组分的相关指数 ,分析其连通性、完整性、破碎化程度及聚集程度。结果表明 :研究区各景观组分分布受海拔高度和坡向的影响非常明显。各景观组分的完整性、连通性和破碎化程度也很不均衡。草地是研究区面积最大、连通性和完整性最好的景观组分 ;青海云杉林呈斑块状或带状分布在阴坡和半阴坡 ,形状最为不规则 ,平均斑块面积小且距离近 ,易受干扰而发生重大变化 ;宜林地和祁连圆柏林相对于农田、疏林地有较强的扩张特征 ;而杨类阔叶林各斑块间相隔距离大 ,斑块之间的邻接性差 ,破碎化程度最为严重。     

西狭文化旅游形象三重结构整合

西狭文化旅游资源是甘肃成县旅游开发的优势资源。由于对西狭文化理解上的不全面 ,导致西狭文化旅游区的建设存在一系列偏差 ,旅游形象不突出。文章提出了西狭文化旅游形象的三重结构整合的思路 ,即文化结构整合、空间结构整合与景观结构整合 ;并且认为 :文化结构整合是西狭文化旅游形象三重结构整合的前提、基础 ;空间结构整合是由文化结构整合向景观结构整合的过渡 ,而景观结构整合是西狭文化旅游形象三重结构整合的关键。     

Quantifying patch distribution at multiple spatial scales: Applications to wildlife-habitat models

Multiscale analyses are widely employed for wildlife-habitat studies. In most cases, however, each scale is considered discrete and little emphasis is placed on incorporating or measuring the responses of wildlife to resources across multiple scales. We modeled the responses of three Arctic wildlife species to vegetative resources distributed at two spatial scales: patches and collections of patches aggregated across a regional area. We defined a patch as a single or homogeneous collection of pixels representing 1 of 10 unique vegetation types. We employed a spatial pattern technique, three-term local quadrat variance, to quantify the distribution of patches at a larger regional scale. We used the distance at which the variance for each of 10 vegetation types peaked to define a moving window for calculating the density of patches. When measures of vegetation patch and density were applied to resource selection functions, the most parsimonious models for wolves and grizzly bears included covariates recorded at both scales. Seasonal resource selection by caribou was best described using a model consisting of only regional scale covariates. Our results suggest that for some species and environments simple patch-scale models may not capture the full range of spatial variation in resources to which wildlife may respond. For mobile animals that range across heterogeneous areas we recommend selection models that integrate resources occurring at a number of spatial scales. Patch density is a simple technique for representing such higher-order spatial patterns.     

Effect of insulin on the secretion of VEGF and its receptor in serum-free cultured bovine thoracic aortic endothelial cells

AIM: This study was designed to investigate the secretion of VEGF and its receptor (flt-1 or flk-1/KDR) protein by cultured bovine thoracic aortic endothelial cells treated with various insulin concentrations. METHODS: Endothelial cells was isolated from bovine thoracic aorta, and cultured in serum-free medium, then incubated with different insulin concentrations (30 mU/L, 300 mU/L, 3 000 mU/L). The level of VEGF and its receptor (flt-1 or flk-1/KDR) protein were detected by immunohistochemical staining. RESULTS: As compared with no insulin group, the expression of VEGF protein in low insulin concentration (30 mU/L and 300 mU/L) groups were significantly increased (P＜0.01). The expression of VEGF protein in high insulin concentration (3 000 mU/L) group was significantly decreased (P＜0.05). Howerer, no difference of the expression of VEGF receptor (flt-1 or flk-1/KDR) protein among all groups (P＞0.05) was observed. CONCLUSION: Low concentration insulin up-regulates the VEGF protein expression while high concentration insulin down-regulates the VEGF protein expression in bovine thoracic aortic endothelial cells, but insulin had no directly effect on the VEGF receptor (flt-1 or flk-1/KDR) protein expression in bovine thoracic aortic endothelial cells.     

当归茎线虫病田间分布型及抽样技术研究

为提高当归茎线虫病(Ditylenchus destructor)田间抽样的准确度,用7种聚集指标判断了当归茎线虫病的田间分布型.结果表明,当归茎线虫病在田间呈聚集分布,其中奈曼分布(核心分布)的适合率为100%,嵌纹分布(负二项分布)的适合率为77.8%.理论抽样数量约820株.4种抽样方法差异均不显著,在保证抽样数量合理的情况下,4种方法均可采用.     

植物化感作用影响因素的再认识

土壤养分、酶、微生物、本底特性,根茬还田、水分、植物根系、种植体系和种植模式与化感作用的互作关系,表明这些因子可通过对土壤养分有效性、微生物种群结构、土壤酶活性和化感物质浓度的影响而对化感作用产生直接或间接影响,这些可为通过化感作用途径构建高效农田生态系统提供新的思路.